PROCEDURE
(for preparation of temporary microscopic slide)
- Small piece of wet sample (Ginger in this experiment) was hold on the left hand.
- The transverse section was cut using the razor blade and was put on a watch glass containing water.
- The finest section was placed on the other watch glass using a brush.
- A drop of safranin stain was put on the section and wait for 30 sec to 2 min.
- The section was transferred back to water using the brush.
- Then, the section was transferred to the slide and and a drop of gylcerin was put on it as a mounting agent.
- This was covered this with the cover slip gently so no air bubble is entrapped.
MICROSCOPICAL FEATURES
- The unpeeled rhizome shows the presence of irregularly arranged cells followed by radially arranged cells.
- This is followed by cortical zone differentiated into outer zone of flattened parenchyma and inner zone of normal parenchyma. The oil cells, oleoresin cells and the cells with starch grains are scattered in the cortical region.
- The innermost cortical region is marked by a single layered endodermis. This is followed by a pericycle layer of stele.
- Inner to that, vascular bundles are present which are collateral and closed. Larger bundles are enclosed by a sheath of non lignified fibres. These vascular bundles resemble to the cortex except for a ring of small bundles scattered immediately within the pericycle.
Good job 👏
ReplyDelete