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Please draw figure on your own based on book, notes provided and what you saw on the microscope.

Wednesday, February 6, 2019

Experiment 2 Study of microscopic features of Ginger

STUDY OF MICROSCOPIC FEATURES OF GINGER

PROCEDURE
(for preparation of temporary microscopic slide)


  • Small piece of wet sample (Ginger in this experiment) was hold on the left  hand.
  • The transverse section was cut using the razor blade and was put on a watch glass containing water.
  • The finest section was placed on the  other watch glass using a brush.
  • A drop of safranin stain was put on the section and wait for 30 sec to 2 min.
  • The section was transferred back to water using the brush.
  • Then,  the section was transferred to the slide and and a drop of gylcerin was put on it as a mounting agent.
  • This was covered  this with the cover slip gently so no air bubble is entrapped.


MICROSCOPICAL FEATURES

  • The unpeeled rhizome shows the presence of irregularly arranged cells followed by radially arranged cells.
  • This is followed by cortical zone differentiated into outer zone of flattened parenchyma and inner zone of normal parenchyma. The oil cells, oleoresin cells and the cells with starch grains are scattered in the cortical region.
  • The innermost cortical region is marked by a single layered endodermis. This is followed by a pericycle layer of stele. 
  • Inner to that, vascular bundles are present which are collateral and closed. Larger bundles are enclosed by a sheath of non lignified fibres. These vascular bundles resemble to the cortex except for a ring of small bundles scattered immediately within the pericycle.

Experiment 1 Study of macroscopical features of Ginger

STUDY OF MACROSCOPICAL CHARACTERISTICS OF GINGER

SYNONYMS: Zingiber, Zingiberis Rhizoma, Aduwa
BIOLOGICAL SOURCE: Ginger is the dried scraped or unscraped rhizomes of Zingiber officinale
ORGANOLEPTIC EVALUATION:
Color: Buff-colored
Odor: Agreeable and aromatic
Taste: Pleasantly pungent and aromatic
Shape: Irregular, flattened
Size (calaculated on the basis of following observation table)
 SN
Length
Breadth
Thickness
 1



 2



 3



 4



 5



Average   ----------------                -----------------                ---------------------
#This table should't be necessarily kept on the file. You can just mention the average value.
Fracture: short and Fibrous

SURFACE CHARACTERS

  • The dried rhizomes are laterally compressed bering short ovate branches on the upper side. Each branch has a depressed scar at its apex.
  • The surface is longitudinally striated with occasional loose fibres.
  • The scraped drug shows no sign of corks or root scars.


USES
# you can also write this part on the experiment regarding microscopical features
  • Ginger is used in the treatment of dyspepsia, flatulence, vomiting, diarrhea and other stomach complaints.
  • Ginger can also be used for the treatment of sorethroat, hoarseness and loss of voice.
  • Ginger is provides relief from cough and asthma.
  • It can also be utilized as flavoring agent in several pharmaceutical preparations.


Lab

Label for Herbarium Field visit Report 2080 Demonstrate different parts of microscope and their application. Study of macroscopical features...